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KMID : 1025520110530010029
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Journal of Animal Science and Technology
2011 Volume.53 No. 1 p.29 ~ p.34
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An Empirical Study on Verifying the Estimated Discrimination and Parentage Test Powers of the 13 Traceability Microsatellite Markers for Commercial Pigs Produced by a Three-way Cross
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Lim Hyun-Tae
Kim Byeong-Woo
Cho In-Cheol
Yoo Chae-Kyoung
Park Moon-Sung
Park Hee-Bok
Lee J.-B.
Lee Jung-Gyu
Jeon Jin-Tae
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Abstract
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Using the materials collected from nine farms in a three-way cross system to produce commercial pigs produced from F1 sows (Landrace×Large White)×Duroc, the power of individual discrimination and parentage of the 13 microsatellite(MS) marker set that has been suggested for individual/brand identification(traceability) was empirically tested. Initially, genotypes of the parental population(F1 sows and Duroc), and commercial pigs were determined and the genotype frequency and polymorphic index were estimated using the Cervus 2.0 program. The probability of identity among genotypes of random individuals, that random half sibs and that of full sib individuals, based on the genotypes from 91 F1 sows and Duroc were expected to be 4.94×10－34, 8.16×10－23 and 2.01×10－08, respectively, using the API-CALC version 1.0 program. When commercial pigs were included, the estimates increased to 3.74×10－35, 5.48×10－25 and 2.96×10－11, respectively. For the empirical verification of the estimated powers of individual discrimination and parentage, the parentage test was performed for 452 commercial pigs using PAPA version 2.0, and individuals with the same genotype were investigated using the Cervus version 2.0 program. Parents for all commercial pigs were successfully estimated and no identical individual was identified in the pedigree. Although the individual discriminating power was not fully verified because of the lack of individuals corresponding with the theoretical power, the 100% efficiency of parentage test was clearly confirmed. Therefore, we believe that the 13 MS marker set in conjunction with management record/information for the pig production kept in a farm/brand should be useful in the pork traceability in a brand unit.
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KEYWORD
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Microsatellite, Identification, Parentage test, Pork traceability
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